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A Comparative Approach to Characterize the Landscape of Host-Pathogen Protein-Protein Interactions
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
A Comparative Approach to Characterize the Landscape of Host-Pathogen Protein-Protein Interactions

A Comparative Approach to Characterize the Landscape of Host-Pathogen Protein-Protein Interactions

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13:56 min

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July 18, 2013

DOI:

10.3791/50404-v

DOI:
10.3791/50404-v

•

13:56 min
July 18, 2013

•

2 Views
, , , ,
1Unité de Génétique, Papillomavirus et Cancer Humain (GPCH),Institut Pasteur , 2Cellule Pasteur,Université Sorbonne Paris Cité, 3Center for Cancer Systems Biology (CCSB), Harvard Medical School, Department of Cancer Biology,Dana Farber Cancer Institute

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This article focuses on the identification of high-confident interaction datasets between host and pathogen proteins using a combination of two orthogonal methods: yeast two-hybrid followed by a high-throughput interaction assay in mammalian cells called HT-GPCA.

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Protein-protein InteractionsHost-pathogen InteractionsComparative InteractomicsYeast Two-hybridGaussia Luciferase Complementation AssayHigh-throughput ScreeningPathogen Strain VariantsProtein Interaction Networks

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Cite this Article

Muller, M., Cassonnet, P., Favre, M., Jacob, Y., Demeret, C. A Comparative Approach to Characterize the Landscape of Host-Pathogen Protein-Protein Interactions. J. Vis. Exp. (77), e50404, doi:10.3791/50404 (2013).

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