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比较方法来表征景观的宿主 - 病原体蛋白 - 蛋白相互作用
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Immunology and Infection
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JoVE Journal Immunology and Infection
A Comparative Approach to Characterize the Landscape of Host-Pathogen Protein-Protein Interactions
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比较方法来表征景观的宿主 - 病原体蛋白 - 蛋白相互作用

English

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11,056 Views

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13:56 min

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July 18, 2013

DOI:

10.3791/50404-v

DOI:
10.3791/50404-v

•

13:56 min
July 18, 2013

•

2 Views
, , , ,
1Unité de Génétique, Papillomavirus et Cancer Humain (GPCH),Institut Pasteur , 2Cellule Pasteur,Université Sorbonne Paris Cité, 3Center for Cancer Systems Biology (CCSB), Harvard Medical School, Department of Cancer Biology,Dana Farber Cancer Institute

Summary

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本文重点介绍高信心之间的相互作用数据库宿主与病原体的蛋白质结合使用两个正交的方法识别：酵母双杂交，然后通过高通量相互作用的检测在哺乳动物细胞中名为HT-GPCA。

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Cite this Article

Muller, M., Cassonnet, P., Favre, M., Jacob, Y., Demeret, C. A Comparative Approach to Characterize the Landscape of Host-Pathogen Protein-Protein Interactions. J. Vis. Exp. (77), e50404, doi:10.3791/50404 (2013).

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