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Isolamento de precursor de células B subconjuntos de Sangue do Cordão Umbilical
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Isolation of Precursor B-cell Subsets from Umbilical Cord Blood

Isolamento de precursor de células B subconjuntos de Sangue do Cordão Umbilical

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17,655 Views

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14:06 min

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April 16, 2013

DOI:

10.3791/50402-v

DOI:
10.3791/50402-v

•

14:06 min
April 16, 2013

•

3 Views
, , , ,
1Department of Pathology and Anatomical Sciences,University of Missouri-Columbia, 2Laboratory for Infectious Disease Research,University of Missouri-Columbia

Summary

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Aqui descrevemos um protocolo para o isolamento de subconjuntos de precursor células B do sangue do cordão umbilical. A quantidade e qualidade suficientes de ácidos nucleicos podem ser extraídos a partir das células e usadas em ensaios subsequentes utilizando DNA ou RNA.

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Cite this Article

Almamun, M., Schnabel, J. L., Gater, S. T., Ning, J., Taylor, K. H. Isolation of Precursor B-cell Subsets from Umbilical Cord Blood. J. Vis. Exp. (74), e50402, doi:10.3791/50402 (2013).

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