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Isolation of Precursor B-cell Subsets from Umbilical Cord Blood
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İmmünoloji ve Enfeksiyon
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JoVE Journal İmmünoloji ve Enfeksiyon
Isolation of Precursor B-cell Subsets from Umbilical Cord Blood

Isolation of Precursor B-cell Subsets from Umbilical Cord Blood

17,655 Views

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14:06 min

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April 16, 2013

DOI:

10.3791/50402-v

DOI:
10.3791/50402-v

•

14:06 min
April 16, 2013

•

3 Views
, , , ,
1Department of Pathology and Anatomical Sciences,University of Missouri-Columbia, 2Laboratory for Infectious Disease Research,University of Missouri-Columbia

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Here we describe a protocol for isolating subsets of precursor B-cells from umbilical cord blood. A sufficient quantity and quality of nucleic acids may be extracted from the cells and used in subsequent assays utilizing DNA or RNA.

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Almamun, M., Schnabel, J. L., Gater, S. T., Ning, J., Taylor, K. H. Isolation of Precursor B-cell Subsets from Umbilical Cord Blood. J. Vis. Exp. (74), e50402, doi:10.3791/50402 (2013).

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