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脑脊液分离解剖大脑皮质外植体使用的啮齿动物胚胎
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Isolation of Cerebrospinal Fluid from Rodent Embryos for use with Dissected Cerebral Cortical Explants
DOI:

09:47 min

March 11, 2013

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Chapters

  • 00:05Title
  • 01:24Micro-capillary Pipette and Embryo Preparations
  • 02:47Ventricular CSF Collection
  • 05:01Cortical Explant Dissection
  • 06:51Transferring and Culturing of Cortical Explant in CSF
  • 08:30CSF Collection Characteristics
  • 09:20Conclusion

Summary

Automatic Translation

心室脑脊液(CSF)沐浴在早期的大脑发育在胚胎神经上皮和大脑皮质祖细胞。在这里,我们描述的方法,从不同年龄层的啮齿动物胚胎隔离室CSF,为了研究其生物学功能。此外,我们展示了我们的脑皮质外植体的解剖和文化的技术,可以为外植体生长的培养基或CSF以最小的体积。

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