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CA同时测量人的心房肌细胞分离<sup> +</sup>瞬变和膜电流
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Isolation of Human Atrial Myocytes for Simultaneous Measurements of Ca
2+
Transients and Membrane Currents
Please note that all translations are automatically generated.
Click here for the English version.
CA同时测量人的心房肌细胞分离<sup> +</sup>瞬变和膜电流
DOI:
10.3791/50235-v
•
10:53 min
•
July 03, 2013
•
Niels Voigt*
1,2
,
Xiao-Bo Zhou*
2
,
Dobromir Dobrev
2
1
Institute of Pharmacology
,
University of Duisburg-Essen
,
2
Division of Experimental Cardiology
,
University of Heidelberg
Chapters
00:05
Title
01:26
Cleaning the Tissue
03:11
Enzymatic Digestions and Precipitation
06:33
Loading of Myocytes with Fluo-3AM
07:53
Representative Cardiomyocytes and Calcium Transient Recordings
10:24
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们描述了人类心房肌细胞可用于细胞内Ca隔离<sup> +</sup>组合测量膜片钳电生理研究。
Tags
Human Atrial Myocytes
Patch-clamp
Ca2+ Transients
Membrane Currents
Atrial Fibrillation
Cell Isolation
Collagenase
Protease
Ca2+ Buffering
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