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Echtzeit-Live-Imaging von T-Zell-Signaling Complex Formation
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Immunology and Infection
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JoVE Journal Immunology and Infection
Real-time Live Imaging of T-cell Signaling Complex Formation
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Echtzeit-Live-Imaging von T-Zell-Signaling Complex Formation

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13,514 Views

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10:31 min

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June 23, 2013

DOI:

10.3791/50076-v

DOI:
10.3791/50076-v

•

10:31 min
June 23, 2013

•

16 Views
, ,
1The Mina and Everard Goodman Faculty of Life Sciences,Bar-Ilan University

Summary

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Wir beschreiben eine Live-Cell-Imaging-Verfahren, das einen Einblick in die Dynamik Protein während der T-Zell-Aktivierung. Wir zeigen die kombinierte Nutzung des T-Zell-Ausbreitung Assay, konfokale Mikroskopie und Bildanalyse zu liefern quantitative Ergebnisse zu folgen Signalisierung Komplexbildung während T-Zell-Aktivierung.

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Cite this Article

Noy, E., Pauker, M. H., Barda-Saad, M. Real-time Live Imaging of T-cell Signaling Complex Formation. J. Vis. Exp. (76), e50076, doi:10.3791/50076 (2013).

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