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December 19, 2012
DOI:
1Department of Radiation Oncology,University of Alabama-Birmingham, 2Department of Radiation Oncology,The Ohio State University Medical School, 3Department of Cell Biology, and Pharmacology and Toxicology, Comprehensive Cancer Center,University of Alabama at Birmingham School of Medicine, University of Alabama-Birmingham
Summary
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O ensaio cometa é uma forma eficiente de detectar quebras simples e de fita dupla, incluindo álcali-lábeis sites e DNA-DNA/DNA-protein ligações cruzadas no DNA em todas as células, incluindo neurônios do hipocampo. O método tira partido da migração diferencial de DNA num campo eléctrico devido a diferenças na quantidade de danos no DNA.
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Cite this Article
Nowsheen, S., Xia, F., Yang, E. S. Assaying DNA Damage in Hippocampal Neurons Using the Comet Assay. J. Vis. Exp. (70), e50049, doi:10.3791/50049 (2012).
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