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Assaying DNA Damage in Hippocampal Neurons Using the Comet Assay
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JoVE Journal 神経科学
Assaying DNA Damage in Hippocampal Neurons Using the Comet Assay

Assaying DNA Damage in Hippocampal Neurons Using the Comet Assay

26,979 Views

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10:00 min

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December 19, 2012

DOI:

10.3791/50049-v

DOI:
10.3791/50049-v

•

10:00 min
December 19, 2012

•

18 Views
, ,
1放射線腫瘍学科,University of Alabama-Birmingham, 2放射線腫瘍学科,The Ohio State University Medical School, 3Department of Cell Biology, and Pharmacology and Toxicology, Comprehensive Cancer Center,University of Alabama at Birmingham School of Medicine, University of Alabama-Birmingham

概要

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The comet assay is an efficient way of detecting single- and double-strand breaks, including alkali-labile sites and DNA-DNA/DNA-protein cross-links on the DNA in all cells including hippocampal neurons. The method takes advantage of the differential migration of DNA in an electric field due to differences in amount of DNA damage.

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Cite this Article

Nowsheen, S., Xia, F., Yang, E. S. Assaying DNA Damage in Hippocampal Neurons Using the Comet Assay. J. Vis. Exp. (70), e50049, doi:10.3791/50049 (2012).

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