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Assaying DNA Damage in Hippocampal Neurons Using the Comet Assay
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JoVE Journal 神経科学
Assaying DNA Damage in Hippocampal Neurons Using the Comet Assay

Assaying DNA Damage in Hippocampal Neurons Using the Comet Assay

DOI:
10.3791/50049-v

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10:00 min

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December 19, 2012

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, ,
1放射線腫瘍学科,University of Alabama-Birmingham, 2放射線腫瘍学科,The Ohio State University Medical School, 3Department of Cell Biology, and Pharmacology and Toxicology, Comprehensive Cancer Center,University of Alabama at Birmingham School of Medicine, University of Alabama-Birmingham

章

  • 00:05標題
  • 02:03Cell Culture
  • 02:51Neutral Comet Assay
  • 05:06Alkaline Comet Assay
  • 06:09Fixing and Staining Cells
  • 06:56Image Acquisition and Analysis
  • 07:31Results: Representative Comet Assays Assessing DNA Damage in Hippocampal Neurons
  • 09:06Conclusion

概要

自動翻訳

自動翻訳

The comet assay is an efficient way of detecting single- and double-strand breaks, including alkali-labile sites and DNA-DNA/DNA-protein cross-links on the DNA in all cells including hippocampal neurons. The method takes advantage of the differential migration of DNA in an electric field due to differences in amount of DNA damage.

タグ

DNA DamageComet AssayDNA RepairSingle Cell Gel ElectrophoresisDNA FragmentationTail MomentTail LengthPercentage DNA In TailDNA Damaging AgentsEtoposide

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