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Imaging Receptor Insertion in die Plasmamembran pHluorin-Tag in der Grundschule Cultured Mäuseneuronen
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November 20, 2012
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PHluorin-tagged Receptor InsertionPlasma MembranePrimary Cultured Mouse NeuronsReceptor TraffickingSpatial ResolutionTemporal ResolutionVesicle Detection SensitivitySurface BiotinylationBiochemical DetectionFluorescence MicroscopyImmunolabeled Surface ReceptorsDynamic Insertion VisualizationSuperecliptic PHluorinPH-sensitive GFP
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Li, Y., Roy, B. D., Wang, W., Zhang, L., Sampson, S. B., Lin, D. Imaging pHluorin-tagged Receptor Insertion to the Plasma Membrane in Primary Cultured Mouse Neurons. J. Vis. Exp. (69), e4450, doi:10.3791/4450 (2012).
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