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Imaging pHluorin-tagged Receptor invoegen in op het plasmamembraan in primaire gekweekte muis neuronen
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Neuroscience
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JoVE Journal Neuroscience
Imaging pHluorin-tagged Receptor Insertion to the Plasma Membrane in Primary Cultured Mouse Neurons
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Imaging pHluorin-tagged Receptor invoegen in op het plasmamembraan in primaire gekweekte muis neuronen

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13,004 Views

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12:58 min

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November 20, 2012

DOI:

10.3791/4450-v

DOI:
10.3791/4450-v

•

12:58 min
November 20, 2012

•

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1The Jackson Laboratory

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PHluorin-tagged Receptor InsertionPlasma MembranePrimary Cultured Mouse NeuronsReceptor TraffickingSpatial ResolutionTemporal ResolutionVesicle Detection SensitivitySurface BiotinylationBiochemical DetectionFluorescence MicroscopyImmunolabeled Surface ReceptorsDynamic Insertion VisualizationSuperecliptic PHluorinPH-sensitive GFP

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Li, Y., Roy, B. D., Wang, W., Zhang, L., Sampson, S. B., Lin, D. Imaging pHluorin-tagged Receptor Insertion to the Plasma Membrane in Primary Cultured Mouse Neurons. J. Vis. Exp. (69), e4450, doi:10.3791/4450 (2012).

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