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Peptide: MHC Tetramero basata Arricchimento di epitopi specifici per cellule T
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Immunology and Infection
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JoVE Journal Immunology and Infection
Peptide:MHC Tetramer-based Enrichment of Epitope-specific T cells
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Peptide: MHC Tetramero basata Arricchimento di epitopi specifici per cellule T

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17,615 Views

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13:58 min

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October 22, 2012

DOI:

10.3791/4420-v

DOI:
10.3791/4420-v

•

13:58 min
October 22, 2012

•

7 Views
,
1Center for Immunology and Inflammatory Diseases, and Pulmonary and Critical Care Unit,Massachusetts General Hospital and Harvard Medical School

Summary

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Questo protocollo descrive l'uso del peptide: MHC tetrameri e microsfere magnetiche per isolare popolazioni bassa frequenza di epitopo-specifiche cellule T e analizzarli mediante citometria a flusso. Questo metodo consente lo studio diretto delle popolazioni di cellule T endogene di interesse da<em> In vivo</em> Sistemi sperimentali.

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Cite this Article

Legoux, F. P., Moon, J. J. Peptide:MHC Tetramer-based Enrichment of Epitope-specific T cells. J. Vis. Exp. (68), e4420, doi:10.3791/4420 (2012).

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