ジャーナル
/
免疫学と感染
/
Differentiating Functional Roles of Gene Expression from Immune and Non-immune Cells in Mouse Colitis by Bone Marrow Transplantation
JoVE Journal
免疫学と感染
このコンテンツを視聴するには、JoVE 購読が必要です。  サインイン又は無料トライアルを申し込む。
JoVE Journal 免疫学と感染
Differentiating Functional Roles of Gene Expression from Immune and Non-immune Cells in Mouse Colitis by Bone Marrow Transplantation

Differentiating Functional Roles of Gene Expression from Immune and Non-immune Cells in Mouse Colitis by Bone Marrow Transplantation

DOI:
10.3791/4208-v

•

14:43 min

•

October 01, 2012

•

, , , ,
1Center for Inflammatory Bowel Diseases, Division of Digestive Diseases, David Geffen School of Medicine,The University of California Los Angeles, Los Angeles

章

  • 00:05標題
  • 01:26Experimental Setup
  • 02:39Donor Bone Marrow Extraction
  • 05:49Infusion to Recipient Mice
  • 07:19Induction of Colitis and Evaluation of Colitis
  • 08:55Quality Inspection of Bone Marrow Transplantation by Flow Cytometry
  • 11:59Transplantation Results
  • 14:21Conclusion

概要

自動翻訳

自動翻訳

Bone marrow transplantation provides a way to change the genotype of the bone marrow derived cells. If the gene of interest is expressed in both bone marrow derived cells and non-bone marrow derived cells, bone marrow transplantation can change the bone marrow derived cells to a different genotype without changing the non-bone marrow derived cell genotype.

タグ

Gene ExpressionImmune CellsNon-immune CellsMouse ColitisBone Marrow TransplantationGene-of-interestKnockout MiceGenotypeIrradiationDonor Bone MarrowRecipient MiceWild-type MiceCD Molecules (CD45.1 And CD45.2)

Article

Embed

プレイリスト追加

Usage Statistics

関連ビデオ

Measuring Calpain Activity in Fixed and Living Cells by Flow Cytometry

Seven Steps to Stellate Cells

Isolation of Functional Cardiac Immune Cells

Isolation, Purification and Labeling of Mouse Bone Marrow Neutrophils for Functional Studies and Adoptive Transfer Experiments

Bone Marrow-derived Macrophage Production

Tracking Mouse Bone Marrow Monocytes In Vivo

Generation and Identification of GM-CSF Derived Alveolar-like Macrophages and Dendritic Cells From Mouse Bone Marrow

Fluorescence-activated Cell Sorting for Purification of Plasmacytoid Dendritic Cells from the Mouse Bone Marrow

Identification and Isolation of Oligopotent and Lineage-committed Myeloid Progenitors from Mouse Bone Marrow

An In Vivo Mouse Model to Measure Naïve CD4 T Cell Activation, Proliferation and Th1 Differentiation Induced by Bone Marrow-derived Dendritic Cells

Read Article