Visualization and Genetic Manipulation of Dendrites and Spines in the Mouse Cerebral Cortex and Hippocampus using In utero Electroporation

Emilie Pacary; Matilda A. Haas; Hendrik Wildner; Roberta Azzarelli; Donald M. Bell; Djoher Nora Abrous; Fran&#231;ois Guillemot

doi:10.3791/4163

Journal

/

Neuroscience

/

可视化和遗传操作在小鼠大脑皮层和使用海马树突和棘在子宫内电穿孔

JoVE Journal
Neuroscience

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JoVE Journal Neuroscience

Visualization and Genetic Manipulation of Dendrites and Spines in the Mouse Cerebral Cortex and Hippocampus using In utero Electroporation

Please note that all translations are automatically generated. Click here for the English version.

可视化和遗传操作在小鼠大脑皮层和使用海马树突和棘在子宫内电穿孔

DOI:

10.3791/4163-v

•

06:30 min

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July 26, 2012

•

Emilie Pacary, Matilda A. Haas, Hendrik Wildner, Roberta Azzarelli, Donald M. Bell, Djoher Nora Abrous, François Guillemot

¹Division of Molecular Neurobiology,MRC National Institute for Medical Research, ²Confocal and Image Analysis Laboratory,National Institute for Medical Research, ³Physiopathologie de la plasticité neuronale, Neurocentre Magendie,Université de Bordeaux

Chapters

00:05Title
01:49Preparation: DNA Solution and Needles
02:10Preparation: Surgery Before DNA Injection and Electroporation
03:25Surgery: DNA Injection and Electroporation
05:01Surgery: Post-surgical Evaluation and Tissue Processing
05:28Representative Results
06:06Conclusion

Summary

Automatic Translation

本文详细介绍了协议，在宫内electroporate大脑皮层和海马小鼠E14.5。我们还表明，这是一个有价值的方法来研究在这两个脑区的树突和刺。

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