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Antibody Transfection into Neurons as a Tool to Study Disease Pathogenesis
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Neurociência
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JoVE Journal Neurociência
Antibody Transfection into Neurons as a Tool to Study Disease Pathogenesis

Antibody Transfection into Neurons as a Tool to Study Disease Pathogenesis

DOI:
10.3791/4154-v

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06:56 min

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September 26, 2012

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, , , , ,
1Research Service,Veterans Administration Medical Center, Memphis, TN, 2Department of Neurology,University of Tennessee Health Science Center, Memphis, TN, 3Department of Anatomy/Neurobiology,University of Tennessee Health Science Center, Memphis, TN

Capítulos

  • 00:05Título
  • 01:17Neuronal Cell Culture
  • 01:39Antibody Labeling
  • 02:48Antibody Transfection
  • 04:18Live and Fixed Imaging to Determine Transfection Efficiency
  • 05:11Results: Live and Fixed Imaging of Antibody-transfected Neurons
  • 05:52Conclusion

Summary

Tadução automática

Tadução automática

A rapid approach to investigate interactions and effects on molecular mechanisms related to the presence of antibodies in an intracellular environment is described. The method involves transfection of antibodies into live cells using a non-covalent complex formation based on a lipid formulation. The technique is adaptable to immortalized cell lines and primary cells.

Tags

Antibody TransfectionNeuronsStudy Disease PathogenesisSpecific AntibodiesHuman DiseasesSystemic Lupus ErythematosusRheumatoid ArthritisParaneoplastic SyndromesMultiple SclerosisHTLV-1Myelopathy/tropical Spastic ParaparesisInflammationCellular MessagingApoptosisIntracellular RNA Binding Protein Heterogeneous Ribonuclear Protein A1 (hnRNP A1)Pathogenic AntibodiesIntracellular Antibody:protein InteractionsTransfection Of AntibodiesAlteration Of Antibody StructurePoor Transfection

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