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Antibody Transfection into Neurons as a Tool to Study Disease Pathogenesis
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September 26, 2012
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A rapid approach to investigate interactions and effects on molecular mechanisms related to the presence of antibodies in an intracellular environment is described. The method involves transfection of antibodies into live cells using a non-covalent complex formation based on a lipid formulation. The technique is adaptable to immortalized cell lines and primary cells.
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Antibody TransfectionNeuronsStudy Disease PathogenesisSpecific AntibodiesHuman DiseasesSystemic Lupus ErythematosusRheumatoid ArthritisParaneoplastic SyndromesMultiple SclerosisHTLV-1Myelopathy/tropical Spastic ParaparesisInflammationCellular MessagingApoptosisIntracellular RNA Binding Protein Heterogeneous Ribonuclear Protein A1 (hnRNP A1)Pathogenic AntibodiesIntracellular Antibody:protein InteractionsTransfection Of AntibodiesAlteration Of Antibody StructurePoor Transfection
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Douglas, J. N., Gardner, L. A., Lee, S., Shin, Y., Groover, C. J., Levin, M. C. Antibody Transfection into Neurons as a Tool to Study Disease Pathogenesis. J. Vis. Exp. (67), e4154, doi:10.3791/4154 (2012).
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