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Mouse Isolotto di Langerhans isolamento utilizzando una combinazione di Collagenasi depurato e proteasi neutra
JoVE Journal
Biology
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JoVE Journal Biology
Mouse Islet of Langerhans Isolation using a Combination of Purified Collagenase and Neutral Protease
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Mouse Isolotto di Langerhans isolamento utilizzando una combinazione di Collagenasi depurato e proteasi neutra

italiano

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42,852 Views

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06:59 min

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September 07, 2012

DOI:

10.3791/4137-v

DOI:
10.3791/4137-v

•

06:59 min
September 07, 2012

•

8 Views
, , , ,
1Department of Pediatrics and the Herman B Wells Center for Pediatric Research,Indiana University School of Medicine, 2VITACYTE, LLC, 3Department of Medicine,Indiana University School of Medicine, 4Department of Cellular and Integrative Physiology,Indiana University School of Medicine

Summary

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Una descrizione dettagliata di isolamento del mouse isolotto è descritta utilizzando la tecnica di incannulazione in situ duttale pancreatico e perfusione di una combinazione di purificato collagenasi e proteasi neutra.

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Cite this Article

Stull, N. D., Breite, A., McCarthy, R., Tersey, S. A., Mirmira, R. G. Mouse Islet of Langerhans Isolation using a Combination of Purified Collagenase and Neutral Protease. J. Vis. Exp. (67), e4137, doi:10.3791/4137 (2012).

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