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Journal / Biology / Mouse Islet of Langerhans Isolation using a…
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Mouse Islet of Langerhans Isolation using a Combination of Purified Collagenase and Neutral Protease
 

Mouse Islet of Langerhans Isolation using a Combination of Purified Collagenase and Neutral Protease

Article DOI: 10.3791/4137-v • 06:59 min • September 7th, 2012
September 7th, 2012 •
1, 2, 2, 1, 1,3,4
1Department of Pediatrics and the Herman B Wells Center for Pediatric Research, Indiana University School of Medicine, 2VITACYTE, LLC, 3Department of Medicine, Indiana University School of Medicine, 4Department of Cellular and Integrative Physiology, Indiana University School of Medicine

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A detailed description of mouse islet isolation is described using the technique of in situ pancreatic ductal cannulation and perfusion of a combination of purified collagenase and neutral protease.

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Mouse Islet Of Langerhans Isolation Purified Collagenase Neutral Protease Beta Cell Gene Expression Intracellular Signaling Pathways Secretory Responses Islet Yield Islet Quality Tissue Dissociating Enzyme (TDE) Rodent Strain Lot-to-lot Variations Dose Of Enzyme Purified TDEs Rodent Cell Isolations Human Islet Isolations VitaCyte LLC Modified Mouse Islet Isolation Protocol Pancreatic Duct Histopaque Gradient
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