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Chapters
- 00:05Title
- 01:27High-throughput Growth of Bacterial Cultures
- 02:38Robotic Purification of Recombinant Proteins
- 04:06Protein Purification
- 06:32Representative Protein Purification Results
- 07:08Conclusion
Tags
High-throughput PurificationAffinity-tagged Recombinant ProteinsX-ray CrystallographyBiochemical AnalysesStructure/function RelationshipsLarge-scale Mutagenesis StrategiesAmino Acids SubstitutionGain/loss-of-function PhenotypesCatalytic ActivityProtein StabilityProtein-protein Interaction SpecificityControlled And Reproducible MannerHigh-throughput StrategiesRobotic Liquid-handling PlatformsHis-tagged Recombinant ProteinsBasal Transcription Machinery
