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High-throughput Purification of Affinity-tagged Recombinant Proteins
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August 26, 2012
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High-throughput PurificationAffinity-tagged Recombinant ProteinsX-ray CrystallographyBiochemical AnalysesStructure/function RelationshipsLarge-scale Mutagenesis StrategiesAmino Acids SubstitutionGain/loss-of-function PhenotypesCatalytic ActivityProtein StabilityProtein-protein Interaction SpecificityControlled And Reproducible MannerHigh-throughput StrategiesRobotic Liquid-handling PlatformsHis-tagged Recombinant ProteinsBasal Transcription Machinery
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Wiesler, S. C., Weinzierl, R. O. High-throughput Purification of Affinity-tagged Recombinant Proteins. J. Vis. Exp. (66), e4110, doi:10.3791/4110 (2012).
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