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Computed Tomography-guided Time-domain Diffuse Fluorescence Tomography in Small Animals for Localization of Cancer Biomarkers
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Medicine
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JoVE Journal Medicine
Computed Tomography-guided Time-domain Diffuse Fluorescence Tomography in Small Animals for Localization of Cancer Biomarkers

Computed Tomography-guided Time-domain Diffuse Fluorescence Tomography in Small Animals for Localization of Cancer Biomarkers

English

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12,043 Views

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12:24 min

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July 17, 2012

DOI:

10.3791/4050-v

DOI:
10.3791/4050-v

•

12:24 min
July 17, 2012

•

9 Views
, , , , , , , ,
1Thayer School of Engineering,Dartmouth College, 2Department of Physics and Astronomy,Dartmouth College, 3Darmouth Medical School,Dartmouth College, 4School of Computer Science,University of Birmingham

Summary

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Diffuse fluorescence tomography offers a relatively low-cost and potentially high-throughout approach to preclinical in vivo tumor imaging. The methodology of optical data collection, calibration, and image reconstruction is presented for a computed tomography-guided non-contact time-domain system using fluorescent targeting of the tumor biomarker epidermal growth factor receptor in a mouse glioma model.

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Cite this Article

Tichauer, K. M., Holt, R. W., Samkoe, K. S., El-Ghussein, F., Gunn, J. R., Jermyn, M., Dehghani, H., Leblond, F., Pogue, B. W. Computed Tomography-guided Time-domain Diffuse Fluorescence Tomography in Small Animals for Localization of Cancer Biomarkers. J. Vis. Exp. (65), e4050, doi:10.3791/4050 (2012).

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