Dergi
/
Tıp
/
Computed Tomography-guided Time-domain Diffuse Fluorescence Tomography in Small Animals for Localization of Cancer Biomarkers
JoVE Journal
Tıp
This content is Free Access.
JoVE Journal Tıp
Computed Tomography-guided Time-domain Diffuse Fluorescence Tomography in Small Animals for Localization of Cancer Biomarkers

Computed Tomography-guided Time-domain Diffuse Fluorescence Tomography in Small Animals for Localization of Cancer Biomarkers

12,043 Views

•

12:24 min

•

July 17, 2012

DOI:

10.3791/4050-v

DOI:
10.3791/4050-v

•

12:24 min
July 17, 2012

•

9 Views
, , , , , , , ,
1Thayer School of Engineering,Dartmouth College, 2Fizik ve Astronomi bölümü,Dartmouth College, 3Darmouth Medical School,Dartmouth College, 4School of Computer Science,University of Birmingham

Özet

Automatically generated

Diffuse fluorescence tomography offers a relatively low-cost and potentially high-throughout approach to preclinical in vivo tumor imaging. The methodology of optical data collection, calibration, and image reconstruction is presented for a computed tomography-guided non-contact time-domain system using fluorescent targeting of the tumor biomarker epidermal growth factor receptor in a mouse glioma model.

Explore More Videos

Computed Tomography-guided Time-domain Diffuse Fluorescence TomographySmall AnimalsCancer BiomarkersPreclinical Drug DiscoveryDevelopment StudiesLight AbsorptionOptical Signal PropagationVisible WavelengthsRed And Near-infrared (near-IR) Light AbsorptionNon-elastic ScatteringFluorescent AgentsImaging SystemsLight Propagation ModelsWhole Body Three-dimensional ImagingIll-posed Nature Of Diffuse Fluorescence TomographyStabilityContrast RecoverySpatial ResolutionImage Reconstruction TechniquesFMI In Small AnimalsCharge-coupled Device (CCD)-based SystemsSensitivity Detectors

Read Article

Cite this Article

Tichauer, K. M., Holt, R. W., Samkoe, K. S., El-Ghussein, F., Gunn, J. R., Jermyn, M., Dehghani, H., Leblond, F., Pogue, B. W. Computed Tomography-guided Time-domain Diffuse Fluorescence Tomography in Small Animals for Localization of Cancer Biomarkers. J. Vis. Exp. (65), e4050, doi:10.3791/4050 (2012).

Download .ris file

Copy

Share Video

.

Copy