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La inmunohistoquímica wholemount de Topografía Revelando Complex Brain
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Neuroscience
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JoVE Journal Neuroscience
Wholemount Immunohistochemistry for Revealing Complex Brain Topography
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La inmunohistoquímica wholemount de Topografía Revelando Complex Brain

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15,427 Views

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09:23 min

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April 05, 2012

DOI:

10.3791/4042-v

DOI:
10.3791/4042-v

•

09:23 min
April 05, 2012

•

4 Views
, , ,
1Department of Neuroscience,Albert Einstein College of Medicine, Yeshiva University , 2Department of Cell Biology and Anatomy and the Hotchkiss Brain Institute, Faculty of Medicine,University of Calgary

Summary

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Circuitos neuronales están topográficamente organizadas en compartimentos funcionales con determinados perfiles moleculares. Aquí, ofrecemos los pasos prácticos y técnicos para revelar la topografía global del cerebro mediante un método versátil wholemount tinción inmunohistoquímica. Se demuestra la utilidad del método que utiliza la citoarquitectura bien entendida y la circuitería de cerebelo.

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White, J. J., Reeber, S. L., Hawkes, R., Sillitoe, R. V. Wholemount Immunohistochemistry for Revealing Complex Brain Topography. J. Vis. Exp. (62), e4042, doi:10.3791/4042 (2012).

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