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Imuno-histoquímica para Topografia Wholemount cerebrais, Complexo
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Neuroscience
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JoVE Journal Neuroscience
Wholemount Immunohistochemistry for Revealing Complex Brain Topography

Imuno-histoquímica para Topografia Wholemount cerebrais, Complexo

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15,427 Views

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09:23 min

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April 05, 2012

DOI:

10.3791/4042-v

DOI:
10.3791/4042-v

•

09:23 min
April 05, 2012

•

4 Views
, , ,
1Department of Neuroscience,Albert Einstein College of Medicine, Yeshiva University , 2Department of Cell Biology and Anatomy and the Hotchkiss Brain Institute, Faculty of Medicine,University of Calgary

Summary

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Circuitos neurais são topograficamente organizados em compartimentos funcionais específicas com perfis moleculares. Aqui, apresentamos os passos práticos e técnicos para revelar topografia cérebro global usando uma abordagem de coloração imuno-histoquímica wholemount versátil. Nós demonstrar a utilidade do método utilizando o citoarquitetura bem compreendida e os circuitos de cerebelo.

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White, J. J., Reeber, S. L., Hawkes, R., Sillitoe, R. V. Wholemount Immunohistochemistry for Revealing Complex Brain Topography. J. Vis. Exp. (62), e4042, doi:10.3791/4042 (2012).

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