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Isolamento de Células endoteliais da veia umbilical humana e sua utilização no estudo de transmigração de Neutrófilos sob condições de fluxo
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August 08, 2012
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IsolationHuman Umbilical Vein Endothelial CellsNeutrophil TransmigrationFlow ConditionsWhite Blood CellInnate Immune SystemAcute InflammationInjury SiteBlood VesselsMicrovascular Structural ChangesPlasma ProteinsRollingAdhesionAccumulationIn Vivo MethodsIn Vitro MethodsHuman NeutrophilsPrimary Human Umbilical Vein Endothelial Cells (HUVEC)Neutrophil IsolationConfluenceActivation MoleculesCytokines
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Ganguly, A., Zhang, H., Sharma, R., Parsons, S., Patel, K. D. Isolation of Human Umbilical Vein Endothelial Cells and Their Use in the Study of Neutrophil Transmigration Under Flow Conditions. J. Vis. Exp. (66), e4032, doi:10.3791/4032 (2012).
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