ジャーナル
/
免疫学と感染
/
Isolation of Human Umbilical Vein Endothelial Cells and Their Use in the Study of Neutrophil Transmigration Under Flow Conditions
JoVE Journal
免疫学と感染
このコンテンツを視聴するには、JoVE 購読が必要です。  サインイン又は無料トライアルを申し込む。
JoVE Journal 免疫学と感染
Isolation of Human Umbilical Vein Endothelial Cells and Their Use in the Study of Neutrophil Transmigration Under Flow Conditions

Isolation of Human Umbilical Vein Endothelial Cells and Their Use in the Study of Neutrophil Transmigration Under Flow Conditions

DOI:
10.3791/4032-v

•

08:54 min

•

August 08, 2012

•

, , , ,
1Department of Physiology and Pharmacology,University of Calgary

章

  • 00:05標題
  • 01:41Isolation and Maintenance of Human Vascular Endothelial Cells
  • 04:54Neutrophil Recruitment and Transmigration
  • 06:36Results: Fluorescent and Cell Migration Analysis of Neutrophil Transmigration Across Endothelial Cells in an Ibidi Chamber
  • 08:03Conclusion

概要

自動翻訳

自動翻訳

This article first describes a procedure for isolating human endothelial cells from umbilical veins and then shows how to use these cells to examine neutrophil transmigration under flow conditions. By using a low-volume flow chamber made from a polymer with the optical characteristics of glass, live-cell fluorescent imaging of rare cell populations is also possible.

タグ

IsolationHuman Umbilical Vein Endothelial CellsNeutrophil TransmigrationFlow ConditionsWhite Blood CellInnate Immune SystemAcute InflammationInjury SiteBlood VesselsMicrovascular Structural ChangesPlasma ProteinsRollingAdhesionAccumulationIn Vivo MethodsIn Vitro MethodsHuman NeutrophilsPrimary Human Umbilical Vein Endothelial Cells (HUVEC)Neutrophil IsolationConfluenceActivation MoleculesCytokines

Article

Embed

プレイリスト追加

Usage Statistics

関連ビデオ

Quantitative In vitro Assay to Measure Neutrophil Adhesion to Activated Primary Human Microvascular Endothelial Cells under Static Conditions

A Flow Adhesion Assay to Study Leucocyte Recruitment to Human Hepatic Sinusoidal Endothelium Under Conditions of Shear Stress

Human Neutrophil Flow Chamber Adhesion Assay

Real-time Imaging of Endothelial Cell-cell Junctions During Neutrophil Transmigration Under Physiological Flow

In Vitro and In Vivo Model to Study Bacterial Adhesion to the Vessel Wall Under Flow Conditions

The Use of Flow Cytometry to Assess the State of Chromatin in T Cells

Neutrophil Isolation and Analysis to Determine their Role in Lymphoma Cell Sensitivity to Therapeutic Agents

Quantification of Monocyte Transmigration and Foam Cell Formation from Individuals with Chronic Inflammatory Conditions

Isolation and In Vitro Culture of Murine and Human Alveolar Macrophages

Simultaneous Study of the Recruitment of Monocyte Subpopulations Under Flow In Vitro

Read Article