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Two Methods of Heterokaryon Formation to Discover HCV Restriction Factors
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Two Methods of Heterokaryon Formation to Discover HCV Restriction Factors

Two Methods of Heterokaryon Formation to Discover HCV Restriction Factors

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16:49 min

July 16, 2012

DOI:

16:49 min
July 16, 2012

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Summary

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We describe two methods for conditional trans-complementation of hepatitis C virus (HCV) assembly and the completion of the full viral life cycle, which rely on heterokaryon formation. These techniques are suitable to screen for cell lines that express dominant restriction factors, which preclude production of infectious HCV progeny.

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