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Two Methods of Heterokaryon Formation to Discover HCV Restriction Factors
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Two Methods of Heterokaryon Formation to Discover HCV Restriction Factors
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July 16, 2012
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We describe two methods for conditional trans-complementation of hepatitis C virus (HCV) assembly and the completion of the full viral life cycle, which rely on heterokaryon formation. These techniques are suitable to screen for cell lines that express dominant restriction factors, which preclude production of infectious HCV progeny.
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Frentzen, A., Hueging, K., Bitzegeio, J., Pietschmann, T., Steinmann, E. Two Methods of Heterokaryon Formation to Discover HCV Restriction Factors. J. Vis. Exp. (65), e4029, doi:10.3791/4029 (2012).
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