/
/
Two Methods of Heterokaryon Formation to Discover HCV Restriction Factors
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich. Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
Two Methods of Heterokaryon Formation to Discover HCV Restriction Factors
11,453 Views
•
16:49 min
•
July 16, 2012
DOI:
Summary
Automatically generated
We describe two methods for conditional trans-complementation of hepatitis C virus (HCV) assembly and the completion of the full viral life cycle, which rely on heterokaryon formation. These techniques are suitable to screen for cell lines that express dominant restriction factors, which preclude production of infectious HCV progeny.
Explore More Videos
Heterokaryon FormationHCV Restriction FactorsHCV ReplicationHost-rangeChimpanzeesHCV RNA ReplicationNon-hepatic Cell LinesMurine Cell LinesSelection ProceduresHCV Replicon ConstructsAntibiotic-selectable MarkersIn Vitro ResearchHepatoma Cell LinesViral Life CycleSpecies TropismImmunocompetent Small Animal ModelGenetic IncompatibilityHost Dependency FactorsRestriction FactorsConditional Trans-complementation MethodsSomatic Cell FusionViral Replication CycleHeterokaryonsTrans-complementationInfectious Viral ProgenySubgenomic HCV Rep
Read Article
Cite this Article
Frentzen, A., Hueging, K., Bitzegeio, J., Pietschmann, T., Steinmann, E. Two Methods of Heterokaryon Formation to Discover HCV Restriction Factors. J. Vis. Exp. (65), e4029, doi:10.3791/4029 (2012).
Copy