This content is Free Access.
11,285 Views
•
08:48 min
•
June 28, 2012
DOI:
Summary
Automatically generated
Genome assemblies based on massively parallel DNA sequencing technologies are usually highly fragmented. The development of physical chromosome maps can potentially improve genome assemblies. Here, we demonstrate innovative approaches to chromosome preparation, fluorescent in situ hybridization, and imaging that significantly increase throughput of the physical map development.
Explore More Videos
High-throughputPhysical MappingChromosomesAutomated In Situ HybridizationWhole-genome SequencesVertebrate SpeciesInsect SpeciesArthropod SpeciesMalaria Mosquito SpeciesIllumina PlatformGenome AssembliesComparative AnalysisEvolutionary ChangesVector AbilityNext-generation Sequencing ReadsDe Novo Genome AssembliesAnopheles GambiaeSanger MethodGapped SequencesFragmented SequencesGenomic AnalysesSequencing ContigsChromosome-based Genome AssembliesUnmapped SequencesGenomic Annotation
Read Article
Cite this Article
George, P., Sharakhova, M. V., Sharakhov, I. V. High-throughput Physical Mapping of Chromosomes using Automated in situ Hybridization. J. Vis. Exp. (64), e4007, doi:10.3791/4007 (2012).
Copy