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High-throughput Physical Mapping of Chromosomes using Automated in situ Hybridization
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June 28, 2012
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Genome assemblies based on massively parallel DNA sequencing technologies are usually highly fragmented. The development of physical chromosome maps can potentially improve genome assemblies. Here, we demonstrate innovative approaches to chromosome preparation, fluorescent in situ hybridization, and imaging that significantly increase throughput of the physical map development.
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High-throughputPhysical MappingChromosomesAutomated In Situ HybridizationWhole-genome SequencesVertebrate SpeciesInsect SpeciesArthropod SpeciesMalaria Mosquito SpeciesIllumina PlatformGenome AssembliesComparative AnalysisEvolutionary ChangesVector AbilityNext-generation Sequencing ReadsDe Novo Genome AssembliesAnopheles GambiaeSanger MethodGapped SequencesFragmented SequencesGenomic AnalysesSequencing ContigsChromosome-based Genome AssembliesUnmapped SequencesGenomic Annotation
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George, P., Sharakhova, M. V., Sharakhov, I. V. High-throughput Physical Mapping of Chromosomes using Automated in situ Hybridization. J. Vis. Exp. (64), e4007, doi:10.3791/4007 (2012).
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