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Low Molecular Weight Protein Enrichment on Mesoporous Silica Thin Films for Biomarker Discovery
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April 17, 2012
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We developed a technology based on mesoporous silica thin film for the selective recovery of low molecular weight proteins and peptides from human serum. The physico-chemical properties of our mesoporous chips were finely tuned to provide substantial control in peptide enrichment and consequently profile the serum proteome for diagnostic purposes.
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Low Molecular Weight Protein EnrichmentMesoporous Silica Thin FilmsBiomarker DiscoveryCirculating BiomarkersEarly DiagnosisPrognosisTherapeutic EfficiencyLow Molecular Weight Proteome (LMWP)Mass Spectrometry (MS)Dynamic RangeProtein And Peptide ConcentrationsSample Pre-treatmentHighly Abundant ProteinsLow-abundance SpeciesProteomic-based ApproachesTwo-dimensional Polyacrylamide Gel-electrophoresis (2D-PAGE)Shotgun Proteomics MethodsClinical ApplicationsIsolation Of LMWP From BloodHigh Throughput ScreeningMulti-fractionation SystemMesoporous Silica Chips
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Fan, J., Gallagher, J. W., Wu, H., Landry, M. G., Sakamoto, J., Ferrari, M., Hu, Y. Low Molecular Weight Protein Enrichment on Mesoporous Silica Thin Films for Biomarker Discovery. J. Vis. Exp. (62), e3876, doi:10.3791/3876 (2012).
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