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Low Molecular Weight Protein Enrichment on Mesoporous Silica Thin Films for Biomarker Discovery
JoVE 杂志
生物工程
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JoVE 杂志 生物工程
Low Molecular Weight Protein Enrichment on Mesoporous Silica Thin Films for Biomarker Discovery

Low Molecular Weight Protein Enrichment on Mesoporous Silica Thin Films for Biomarker Discovery

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13:00 min

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April 17, 2012

DOI:

10.3791/3876-v

DOI:
10.3791/3876-v

•

13:00 min
April 17, 2012

•

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1Department of Nanomedicine,The Methodist Hospital Research Institute, 2CAS Key Laboratory for Biological Effects of Nanomaterials & Nanosafety,National Center for Nanoscience and Technology

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We developed a technology based on mesoporous silica thin film for the selective recovery of low molecular weight proteins and peptides from human serum. The physico-chemical properties of our mesoporous chips were finely tuned to provide substantial control in peptide enrichment and consequently profile the serum proteome for diagnostic purposes.

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Low Molecular Weight Protein EnrichmentMesoporous Silica Thin FilmsBiomarker DiscoveryCirculating BiomarkersEarly DiagnosisPrognosisTherapeutic EfficiencyLow Molecular Weight Proteome (LMWP)Mass Spectrometry (MS)Dynamic RangeProtein And Peptide ConcentrationsSample Pre-treatmentHighly Abundant ProteinsLow-abundance SpeciesProteomic-based ApproachesTwo-dimensional Polyacrylamide Gel-electrophoresis (2D-PAGE)Shotgun Proteomics MethodsClinical ApplicationsIsolation Of LMWP From BloodHigh Throughput ScreeningMulti-fractionation SystemMesoporous Silica Chips

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Fan, J., Gallagher, J. W., Wu, H., Landry, M. G., Sakamoto, J., Ferrari, M., Hu, Y. Low Molecular Weight Protein Enrichment on Mesoporous Silica Thin Films for Biomarker Discovery. J. Vis. Exp. (62), e3876, doi:10.3791/3876 (2012).

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