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使用人工痰介质测试抗生素药效试验<em>绿脓杆菌</em>更多相关的肺囊肿性纤维化的条件
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Use of Artificial Sputum Medium to Test Antibiotic Efficacy Against Pseudomonas aeruginosa in Conditions More Relevant to the Cystic Fibrosis Lung
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使用人工痰介质测试抗生素药效试验<em>绿脓杆菌</em>更多相关的肺囊肿性纤维化的条件

English

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31,982 Views

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07:46 min

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June 05, 2012

DOI:

10.3791/3857-v

DOI:
10.3791/3857-v

•

07:46 min
June 05, 2012

•

22 Views
, , , , ,
1Institute of Infection and Global Health,University of Liverpool , 2NIHR Biomedical Research Centre in Microbial Disease,University of Liverpool

Summary

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目前的诊断药敏试验依靠浮游株生长在营养丰富，有氧条件下的。在这里，我们采用了一种替代人工痰媒介研究的肺囊肿性纤维化的代表都需氧和微需氧条件下绿脓杆菌生物膜药敏。

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Artificial Sputum MediumAntibiotic EfficacyPseudomonas AeruginosaCystic Fibrosis LungIn Vitro Antimicrobial Susceptibility TestsMicroaerophilic ConditionsBiofilmsArtificial Sputum Medium AssayMicrotitre-plate AssayAntimicrobial Susceptibility

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Cite this Article

Kirchner, S., Fothergill, J. L., Wright, E. A., James, C. E., Mowat, E., Winstanley, C. Use of Artificial Sputum Medium to Test Antibiotic Efficacy Against Pseudomonas aeruginosa in Conditions More Relevant to the Cystic Fibrosis Lung. J. Vis. Exp. (64), e3857, doi:10.3791/3857 (2012).

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