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Use of Artificial Sputum Medium to Test Antibiotic Efficacy Against Pseudomonas aeruginosa in Conditions More Relevant to the Cystic Fibrosis Lung
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Use of Artificial Sputum Medium to Test Antibiotic Efficacy Against Pseudomonas aeruginosa in Conditions More Relevant to the Cystic Fibrosis Lung
Bölümler
- 00:05Başlık
- 01:24Preparation of Artificial Sputum Medium (ASM) for Biofilm Cultures
- 03:14Determination of Biofilm Sessile Cell Minimum Inhibitory Concentration (BSMIC)
- 06:22Results: Properties and Antibacterial Resistance of P. aeruginosa Biofilms or Planktonic Cultures
- 07:17Conclusion
Current diagnostic antimicrobial susceptibility testing relies on the planktonic growth of isolates in nutrient rich, aerobic conditions. Here, we employ an alternative artificial sputum medium to study antimicrobial susceptibility of Pseudomonas aeruginosa biofilms under both aerobic and microaerophilic conditions more representative of the cystic fibrosis lung.
