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February 15, 2012
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Mycobacterium tuberculosis forma biofilmes de drogas tolerantes quando cultivadas em certas condições. Aqui descrevemos métodos para cultura de M. biofilmes de tuberculose e que determinam a freqüência de persistentes drogas tolerantes. Estes protocolos serão úteis para estudos posteriores sobre os mecanismos de tolerância à droga em M. tuberculose.
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Mycobacterium TuberculosisGrowthBiofilmsStress ToleranceAntibioticsSurvivalMolecular MechanismsPathogenSelf-assemblingSurface AttachedMatrix Encapsulated StructuresBiofilm GrowthCommunitiesGenetic ComponentsSurface AttachmentIntercellular CommunicationsExtracellular Polymeric Substances (EPS)Environmental Stress TolerancePhysiological AdaptationBacilliComplex ArchitectureAnti-tuberculosis DrugsIsoniazidRifampicin
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Cite this Article
Kulka, K., Hatfull, G., Ojha, A. K. Growth of Mycobacterium tuberculosis Biofilms. J. Vis. Exp. (60), e3820, doi:10.3791/3820 (2012).
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