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Growth of Mycobacterium tuberculosis Biofilms
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February 15, 2012
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Mycobacterium tuberculosis forms drug tolerant biofilms when cultured in certain conditions. Here we describe methods for culturing M. tuberculosis biofilms and determining the frequency of drug tolerant persisters. These protocols will be useful for further studies into the mechanisms of drug tolerance in M. tuberculosis.
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Mycobacterium TuberculosisGrowthBiofilmsStress ToleranceAntibioticsSurvivalMolecular MechanismsPathogenSelf-assemblingSurface AttachedMatrix Encapsulated StructuresBiofilm GrowthCommunitiesGenetic ComponentsSurface AttachmentIntercellular CommunicationsExtracellular Polymeric Substances (EPS)Environmental Stress TolerancePhysiological AdaptationBacilliComplex ArchitectureAnti-tuberculosis DrugsIsoniazidRifampicin
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Kulka, K., Hatfull, G., Ojha, A. K. Growth of Mycobacterium tuberculosis Biofilms. J. Vis. Exp. (60), e3820, doi:10.3791/3820 (2012).
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