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Den neuroblast Assay: Et assay for generering og berigelse af neuronale progenitorceller fra Differentiering neural stamcelle Afkom anvendelse af flowcytometri
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Chapters
- 00:02Title
- 01:46Starting Neuroblast Assay Culture
- 04:07Switching Neuroblast Assay Medium
- 04:55Cell Preparation for Flow Cytometry
- 06:42Staining for PSA-NCAM
- 08:31Isolation of Neuronal Progenitor Cells Using Flow Cytometry
- 11:34Plating Sorted Cells
- 13:12Representative Results
- 14:34Conclusion
Denne video protokol viser en hidtil ukendt fremgangsmåde til generering og efterfølgende oprensning af neuronale progenitorceller fra en fornyelig kilde af neurale stamceller (NSC) baseret på deres fysiske (størrelse og intern granularitet) og fluorescerende egenskaber ved hjælp af flowcytometri-teknologi.
Tags
Neuroblast AssayGeneration Of Neuronal Progenitor CellsDifferentiating Neural Stem CellsFlow CytometryNeurosphere AssayCentral Nervous SystemAstrocytesOligodendrocytesNeuronsIn Vitro StudiesDrug ScreeningNeurotoxicologyElectrophysiologyCell Replacement TherapyHeterogeneity Of NSC ProgenyLow Production Of Neurons And OligodendrocytesPredominance Of AstrocytesClinical ApplicationsPurification Of Immature NeuronsFluorescence Activated Cell Sorting (FACS)Murine NSC ProgenyE14 Mouse Ganglionic Eminences
