Journal
/
Neurociencias
/
DiI-Labeling of DRG Neurons to Study Axonal Branching in a Whole Mount Preparation of Mouse Embryonic Spinal Cord
JoVE Revista
Neurociencias
Se requiere una suscripción a JoVE para ver este contenido.  Inicie sesión o comience su prueba gratuita.
JoVE Revista Neurociencias
DiI-Labeling of DRG Neurons to Study Axonal Branching in a Whole Mount Preparation of Mouse Embryonic Spinal Cord

DiI-Labeling of DRG Neurons to Study Axonal Branching in a Whole Mount Preparation of Mouse Embryonic Spinal Cord

DOI:
10.3791/3667-v

•

09:14 min

•

December 13, 2011

•

,
1Developmental Neurobiology,Max Delbrück Center for Molecular Medicine

Capítulos

  • 00:05Título
  • 01:56Dissection Procedure
  • 04:32DiI Labeling of DRG Neurons
  • 06:29Mounting and Microscopic Analysis
  • 07:27Results: Visualizing and Quantification of Single Axonal Trajectories of Embryonic Mouse DRG Neurons
  • 08:33Conclusion

Summary

Traducción Automática

Traducción Automática

The stereotyped projections of sensory afferents into the rodent spinal cord offer an easily accessible experimental system to study axonal branching through the tracing of single axons.

Tags

DiI-labelingDRG NeuronsAxonal BranchingWhole Mount PreparationMouse Embryonic Spinal CordDiffusive StainingLipophilic Tracer11′-dioctadecyl-333′3′-tetramethylindocarbocyanine Perchlorate (DiI)Axonal PathwaysWild-typeMutated Mouse LinesFunctional RoleCandidate ProteinsControl Of Axonal BranchingWiring Of The Nervous SystemParallel Processing Of InformationIntermediate Target RegionsTerminal ArborizationAxonal GrowthGrowth ConeSplitting BranchingDelayed BranchingInterstitial BranchingCentral Projections Of NeuronsDorsal Root Entry Zone (DREZ)Stereotyped Pattern

Article

Embed

Añadir a la lista de reproducción

Usage Statistics

Videos relacionados

check_url/es/3667?article_type=v

Dissection and Culture of Commissural Neurons from Embryonic Spinal Cord
check_url/es/3667?article_type=v

Visualization of the Embryonic Nervous System in Whole-mount Drosophila Embryos
check_url/es/3667?article_type=v

Whole Mount Preparation of the Adult Drosophila Ventral Nerve Cord for Giant Fiber Dye Injection
check_url/es/3667?article_type=v

An Ex Vivo Laser-induced Spinal Cord Injury Model to Assess Mechanisms of Axonal Degeneration in Real-time
check_url/es/3667?article_type=v

Preparation of Primary Mixed Glial Cultures from Adult Mouse Spinal Cord Tissue
check_url/es/3667?article_type=v

Zebrafish In Situ Spinal Cord Preparation for Electrophysiological Recordings from Spinal Sensory and Motor Neurons
check_url/es/3667?article_type=v

Visualization of the Axonal Projection Pattern of Embryonic Motor Neurons in Drosophila
check_url/es/3667?article_type=v

Laminectomy and Spinal Cord Window Implantation in the Mouse
check_url/es/3667?article_type=v

Preparation of Acute Spinal Cord Slices for Whole-cell Patch-clamp Recording in Substantia Gelatinosa Neurons
check_url/es/3667?article_type=v

Intra-Arterial Delivery of Neural Stem Cells to the Rat and Mouse Brain: Application to Cerebral Ischemia

Read Article