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免疫组化标记在感觉神经元树突的微管，气管，并在肌肉果蝇幼虫体壁
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Neuroscience
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JoVE Journal Neuroscience
Immunohistological Labeling of Microtubules in Sensory Neuron Dendrites, Tracheae, and Muscles in the Drosophila Larva Body Wall
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免疫组化标记在感觉神经元树突的微管，气管，并在肌肉果蝇幼虫体壁

DOI:
10.3791/3662-v

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07:49 min

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November 10, 2011

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, ,
1Disease Mechanism Research Core,RIKEN Brain Science Institute, 2Graduate School of Science and Engineering,Saitama University

Chapters

  • 00:05Title
  • 01:11Larval Dissection
  • 02:56Fixation of Larval Fillets
  • 03:59Blocking, Staining, and Mounting of Larval Fillets
  • 05:56Fluorescent Staining
  • 07:22Conclusion

Summary

Automatic Translation

Automatic Translation

要理解的复杂的细胞形状，如神经细胞树突，如何在发展过程中取得的，重要的是要能够准确地检测微管组织。在这里，我们描述了一个强大的免疫组化标记法研究微管组织的感觉神经元树突树突分枝，气管，肌肉，及其他<em>果蝇</em>幼虫体壁组织。

Tags

Immunohistological LabelingMicrotubulesSensory Neuron DendritesTracheaeMusclesDrosophila Larva Body WallCell ShapesMicrotubule OrganizationTube MorphogenesisDendritic ArborizationNeuron-class-specific MechanismsDendritic DifferentiationDegenerationCytoskeletal OrganizationImmunological Labeling MethodIn Vivo Microtubule OrganizationFirst Instar LarvaSensory Neuron Dendrite OutgrowthBranching OrganizationMuscles LabelingTrachea Labeling

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