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Use of Interferon-γ Enzyme-linked Immunospot Assay to Characterize Novel T-cell Epitopes of Human Papillomavirus
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Use of Interferon-γ Enzyme-linked Immunospot Assay to Characterize Novel T-cell Epitopes of Human Papillomavirus
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13:41 min
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March 08, 2012
DOI:
1Department of Microbiology and Parasitology, College of Basic Medical Sciences,China Medical University , 2Department of Obstetrics and Gynecology, College of Medicine,University of Arkansas for Medical Sciences , 3Department of Pathology, College of Medicine,University of Arkansas for Medical Sciences
Summary
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Characterizing T-cell epitopes of pathogens that cause localized infections such as human papillomavirus is a challenge because of limited number of T cells in circulation. A method is described in which rare T cells were isolated and were characterized starting with a very small number of cells.
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Cite this Article
Wang, X., Greenfield, W. W., Coleman, H. N., James, L. E., Nakagawa, M. Use of Interferon-γ Enzyme-linked Immunospot Assay to Characterize Novel T-cell Epitopes of Human Papillomavirus. J. Vis. Exp. (61), e3657, doi:10.3791/3657 (2012).
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