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July 26, 2012
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Forniamo un protocollo per la coltura di neuroni dell'ippocampo altamente purificato da cervelli dei topi prenatale senza l'uso di uno strato di cellule gliali alimentatore.
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IsolationCultureHippocampal NeuronsPrenatal MicePrimary CulturesRatMurineNeurobiologyCellular MechanismsCellular TraffickingCellular StructureProtein LocalizationBiochemical TechniquesMemoryLearningContaminationBrain Cell TypesSpecific MediaNeuron GrowthDissection TechniquesEmbryonic Day 19 (E19)Mouse PupsTrypsinMechanical DisruptionPipettes
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Seibenhener, M. L., Wooten, M. W. Isolation and Culture of Hippocampal Neurons from Prenatal Mice. J. Vis. Exp. (65), e3634, doi:10.3791/3634 (2012).
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