/

/

Isolation and Culture of Hippocampal Neurons from Prenatal Mice

JoVE 杂志
神经科学

需要订阅 JoVE 才能查看此. 登录或开始免费试用。

JoVE 杂志神经科学

Isolation and Culture of Hippocampal Neurons from Prenatal Mice

Isolation and Culture of Hippocampal Neurons from Prenatal Mice

DOI:

•

10:27 min

•

July 26, 2012

•

Michael L. Seibenhener, Marie W. Wooten

¹Department of Biological Sciences,Auburn University

Chapters

00:05Title
01:12Set-up Prior to Harvest
02:09Tissue Harvest
04:03Tissue Dissociation
05:23Neuron Trituration
06:42Cell Plating
08:43Immunofluorescence Results with Hippocampal Neuronal Cultures
09:58Conclusion

Summary

自动翻译

We provide a protocol for the culture of highly purified hippocampal neurons from prenatal mouse brains without the use of a feeder glial cell layer.

Article

Embed

添加到播放列表

Usage Statistics

Related Videos

Dissection and Culture of Commissural Neurons from Embryonic Spinal Cord

Nucleofection and Primary Culture of Embryonic Mouse Hippocampal and Cortical Neurons

Calcium Phosphate Transfection of Primary Hippocampal Neurons

Isolation and Culture of Dissociated Sensory Neurons From Chick Embryos

Isolation, Culture and Long-Term Maintenance of Primary Mesencephalic Dopaminergic Neurons From Embryonic Rodent Brains

Low-Density Primary Hippocampal Neuron Culture

Spinal Cord Neurons Isolation and Culture from Neonatal Mice

Generation of Neurospheres from Mixed Primary Hippocampal and Cortical Neurons Isolated from E14-E16 Sprague Dawley Rat Embryo

Primary Culture of Neurons Isolated from Embryonic Mouse Cerebellum

Isolation and Culture of Oculomotor, Trochlear, and Spinal Motor Neurons from Prenatal Isl^mn:GFP Transgenic Mice

Read Article