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De alto rendimiento Método de detección de virus de la influenza
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February 04, 2012
DOI:
1Laboratory of Molecular Immunology and Immunotherapy,Blood Research Institute, 2Department of Microbiology,Mount Sinai School of Medicine , 3Laboratory of Molecular Genetics,Blood Research Institute, 4City of Milwaukee Health Department Laboratory, 5Division of Hematology-Oncology/BMT, Children’s Hospital of Wisconsin,Medical College of Wisconsin , 6Division of Hematology and Oncology, Dept Medicine,Medical College of Wisconsin
Summary
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Este método describe el uso de infrarrojos sistema colorante formación de imágenes basada para la detección de H1N1 en bronquioalveolar lavado (BAL) de líquidos de los ratones infectados en una alta sensibilidad. Esta metodología se puede realizar en un 96 - o 384-así placa, requiere <10 l de volumen material de prueba y tiene el potencial para el cribado simultáneo de múltiples patógenos.
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Cite this Article
Kumar, P., Bartoszek, A. E., Moran, T. M., Gorski, J., Bhattacharyya, S., Navidad, J. F., Thakar, M. S., Malarkannan, S. High-throughput Detection Method for Influenza Virus. J. Vis. Exp. (60), e3623, doi:10.3791/3623 (2012).
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