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High-throughput Metodo di rilevazione per il virus dell'influenza
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February 04, 2012
DOI:
1Laboratory of Molecular Immunology and Immunotherapy,Blood Research Institute, 2Department of Microbiology,Mount Sinai School of Medicine , 3Laboratory of Molecular Genetics,Blood Research Institute, 4City of Milwaukee Health Department Laboratory, 5Division of Hematology-Oncology/BMT, Children’s Hospital of Wisconsin,Medical College of Wisconsin , 6Division of Hematology and Oncology, Dept Medicine,Medical College of Wisconsin
Summary
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Questo metodo descrive l'utilizzo di Infrared sistema colorante di imaging based per il rilevamento di H1N1 in bronchioalveolar lavaggio (BAL) fluido di topi infettati ad una sensibilità elevata. Questa metodologia può essere eseguita in uno 96 - o 384-bene piastra, richiede <10 volume pl di materiale di prova e ha il potenziale per lo screening concomitante di agenti patogeni multipli.
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Cite this Article
Kumar, P., Bartoszek, A. E., Moran, T. M., Gorski, J., Bhattacharyya, S., Navidad, J. F., Thakar, M. S., Malarkannan, S. High-throughput Detection Method for Influenza Virus. J. Vis. Exp. (60), e3623, doi:10.3791/3623 (2012).
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