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Directed Cellular Self-Assembly to Fabricate Cell-Derived Tissue Rings for Biomechanical Analysis and Tissue Engineering
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Chapters
- 00:05Title
- 01:13Cell Seeding Mold Fabrication
- 02:41Cell Culture and Ring Seeding
- 03:35Tissue Ring Harvesting and Thickness Measurements
- 04:02Mechanical Testing of Rings
- 04:47Tissue Ring Assembly to Fabricate Tissue Tubes
- 07:12Conclusion
This article outlines a versatile method to create cell-derived tissue rings by cellular self-assembly. Smooth muscle cells seeded into ring-shaped agarose wells aggregate and contract to form robust three-dimensional (3D) tissues within 7 days. Millimeter-scale tissue rings are conducive to mechanical testing and serve as building blocks for tissue assembly.
