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Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy
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13:48 min
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May 29, 2012
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1Department of Thoracic and Cardiovascular Surgery and Inter-University Centre for Medical Technology Stuttgart-Tübingen (IZST),Eberhard Karls University, Tübingen, 2Department of Cell and Tissue Engineering,Fraunhofer Institute of Interfacial Engineering and Biotechnology (IGB) Stuttgart, Germany, 3Department for Medical Interfacial Engineering (IGVT),University of Stuttgart, Germany, 4Institute of Tissue Engineering and Regenerative Medicine,Julius-Maximillians University, Würzburg, Germany
Summary
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Raman spectroscopy is a suitable technique for the non-contact, label-free analysis of living cells, tissue-engineered constructs and native tissues. Source-specific spectral fingerprints can be generated and analyzed using multivariate analysis.
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Raman SpectroscopyNon-contact MonitoringLabel-free MonitoringCellsExtracellular MatrixBiomedical ResearchNon-destructiveLaser-based TechnologySpectral PatternChemical VibrationRaman BandPeak IntensitiesMultivariate AnalysisPrincipal Component Analysis (PCA)Living CellsGlass Bottom DishesCell Culture ConditionsCell NucleusECM Proteins
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Cite this Article
Votteler, M., Carvajal Berrio, D. A., Pudlas, M., Walles, H., Schenke-Layland, K. Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy. J. Vis. Exp. (63), e3977, doi:10.3791/3977 (2012).
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