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在复杂的临床和环境样本使用寡核苷酸加上荧光微多元检测细菌
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Immunology and Infection
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JoVE Journal Immunology and Infection
Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres
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在复杂的临床和环境样本使用寡核苷酸加上荧光微多元检测细菌

DOI:
10.3791/3344-v

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11:09 min

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October 23, 2011

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, , , ,
1Saskatoon Research Centre,Agriculture and Agri-Food Canada, 2Department of Veterinary Microbiology,University of Saskatchewan , 3Plant Biotechnology Institute,National Research Council of Canada

Chapters

  • 00:05Title
  • 03:05Coupling Oligonucleotide Probes to Fluorescent Beads
  • 06:37Production of cpn60 Universal Target Amplicon and Generation of Single Strands
  • 09:36Representative Results

Summary

Automatic Translation

Automatic Translation

我们描述了一个复用的微生物样本内使用寡核苷酸耦合的荧光珠的检测方法。从样本内的所有生物体的扩增杂交的探针耦合珠小组。一个Luminex公司或生物复杂的仪器是用于查询每个珠珠型和杂交信号。

Tags

Multiplex DetectionBacteriaClinical SamplesEnvironmental SamplesOligonucleotide-coupled Fluorescent MicrospheresBacterial VaginosisPolymicrobial SyndromeLactobacillusGardnerella VaginalisAtopobium VaginaeNegative Health OutcomesHIV AcquisitionClinical ManagementDiagnosisGram StainsVaginal Swab SmearsSubjective InterpretationsVaginal Microbiota CompositionDeep SequencingMolecular Diagnosis

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