/
/
Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres
Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.
Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres
16,057 Views
•
11:09 min
•
October 23, 2011
DOI:
Summary
Automatically generated
We describe a multiplex method for the detection of microorganisms within a sample using oligonucleotide-coupled fluorescent beads. Amplicon from all organisms within a sample is hybridized to a panel of probe-coupled beads. A Luminex or Bio-Plex instrument is used to query each bead for bead type and hybridization signal.
Explore More Videos
Multiplex DetectionBacteriaClinical SamplesEnvironmental SamplesOligonucleotide-coupled Fluorescent MicrospheresBacterial VaginosisPolymicrobial SyndromeLactobacillusGardnerella VaginalisAtopobium VaginaeNegative Health OutcomesHIV AcquisitionClinical ManagementDiagnosisGram StainsVaginal Swab SmearsSubjective InterpretationsVaginal Microbiota CompositionDeep SequencingMolecular Diagnosis
Read Article
Cite this Article
Dumonceaux, T. J., Town, J. R., Hill, J. E., Chaban, B. L., Hemmingsen, S. M. Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres. J. Vis. Exp. (56), e3344, doi:10.3791/3344 (2011).
Copy