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Constructing a Low-budget Laser Axotomy System to Study Axon Regeneration in C. elegans
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November 15, 2011
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Laser axotomy followed by time-lapse imaging is a sensitive way to assay the effects of mutations in C. elegans on axon regeneration. A high quality, but inexpensive, laser ablation system can be easily added to most microscopes. Time lapse imaging over 15 hours requires careful immobilization of the worm.
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Low-budgetLaser Axotomy SystemStudyAxon RegenerationC. ElegansAssayCostTechnical ExpertiseLaser Ablation SystemSolid-state Pulse LasersTransparent PreparationsConstructionAlignmentOptical PathFocused CondenserIntermediate ModuleDichroicImagingLaser BeamMicroscope Condenser LensObjective LensFinal AlignmentTesting
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Williams, W., Nix, P., Bastiani, M. Constructing a Low-budget Laser Axotomy System to Study Axon Regeneration in C. elegans. J. Vis. Exp. (57), e3331, doi:10.3791/3331 (2011).
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