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Constructing a Low-budget Laser Axotomy System to Study Axon Regeneration in C. elegans
JoVE Journal
Bioengineering
This content is Free Access.
JoVE Journal Bioengineering
Constructing a Low-budget Laser Axotomy System to Study Axon Regeneration in C. elegans

Constructing a Low-budget Laser Axotomy System to Study Axon Regeneration in C. elegans

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10:05 min

November 15, 2011

DOI:

10:05 min
November 15, 2011

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Summary

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Laser axotomy followed by time-lapse imaging is a sensitive way to assay the effects of mutations in C. elegans on axon regeneration. A high quality, but inexpensive, laser ablation system can be easily added to most microscopes. Time lapse imaging over 15 hours requires careful immobilization of the worm.

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