This content is Free Access.
Constructing a Low-budget Laser Axotomy System to Study Axon Regeneration in C. elegans
15,094 Views
•
10:05 min
•
November 15, 2011
DOI:
概要
Automatically generated
Laser axotomy followed by time-lapse imaging is a sensitive way to assay the effects of mutations in C. elegans on axon regeneration. A high quality, but inexpensive, laser ablation system can be easily added to most microscopes. Time lapse imaging over 15 hours requires careful immobilization of the worm.
Explore More Videos
Low-budgetLaser Axotomy SystemStudyAxon RegenerationC. ElegansAssayCostTechnical ExpertiseLaser Ablation SystemSolid-state Pulse LasersTransparent PreparationsConstructionAlignmentOptical PathFocused CondenserIntermediate ModuleDichroicImagingLaser BeamMicroscope Condenser LensObjective LensFinal AlignmentTesting
Read Article
Cite this Article
Williams, W., Nix, P., Bastiani, M. Constructing a Low-budget Laser Axotomy System to Study Axon Regeneration in C. elegans. J. Vis. Exp. (57), e3331, doi:10.3791/3331 (2011).
Copy