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Analysis of Trunk Neural Crest Cell Migration using a Modified Zigmond Chamber Assay
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Neuroscience
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JoVE Journal Neuroscience
Analysis of Trunk Neural Crest Cell Migration using a Modified Zigmond Chamber Assay

Analysis of Trunk Neural Crest Cell Migration using a Modified Zigmond Chamber Assay

English

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12,338 Views

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12:17 min

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January 19, 2012

DOI:

10.3791/3330-v

DOI:
10.3791/3330-v

•

12:17 min
January 19, 2012

•

2 Views
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1Department of Biology,California State University, Northridge, 2Centro de Biología Celular y Molecular,Universidad Nacional de Córdoba

Summary

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An approach to analyze the migration of explanted cells (trunk neural crest cells) is described. This method is inexpensive, gentle, and capable of distinguishing chemotaxis from both chemokinesis and other influences on migratory polarity such as those derived from cell-cell interactions within the primary trunk neural crest cell culture.

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Cite this Article

Walheim, C. C., Zanin, J. P., de Bellard, M. E. Analysis of Trunk Neural Crest Cell Migration using a Modified Zigmond Chamber Assay. J. Vis. Exp. (59), e3330, doi:10.3791/3330 (2012).

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